- Title
- Evaluation of real-time PCR for diagnosis of Post-kala-azar dermal leishmaniasis in endemic foci of Bangladesh
- Creator
- Ghosh, Prakash; Hasnain, Md Golam; Hossain, Faria; Khan, Md Anik Ashfaq; Chowdhury, Rajashree; Faisal, Khaledul; Mural, Moshtaq Ahmed; Baker, James; Nath, Rupen; Ghosh, Debashis; Maruf, Shomik; Shomik, Mohammad Sohel; Haque, Rashidul; Matlashewski, Greg; Hamano, Shinjiro; Duthie, Malcolm S; Mondal, Dinesh
- Relation
- Open Forum Infectious Diseases Vol. 5, Issue 10, no. ofy234
- Publisher Link
- http://dx.doi.org/10.1093/ofid/ofy234
- Publisher
- Oxford University Press
- Resource Type
- journal article
- Date
- 2018
- Description
- Background: Post-kala-azar dermal leishmaniasis (PKDL) is a sequel to visceral leishmaniasis (VL), which is found in VL-endemic countries including Bangladesh. Because of these enigmatic cases, the success of the National Kala-azar Elimination Program is under threat. To date, diagnostic methods for PKDL cases in endemic regions have been limited to clinical examination and rK39 test or microscopy, and a suitable and accurate alternative method is needed. In this study, we investigated the application of real-time polymerase chain reaction (PCR) as a potential method for diagnosis of PKDL in comparison with microscopy. Methods: Ninety-one suspected macular PKDL cases from Mymensingh district, Bangladesh, were enrolled in the study after diagnosis by clinical examination and an rK39 strip test. All of them responded after completion of the treatment with miltefosine. During enrollment, a skin biopsy was done for each patient, and both microscopy and real-time PCR were performed for detection and quantification of Leishmania donovan body (LDB) and LD DNA, respectively. Results: Real-time PCR detected 83 cases among all suspected PKDL patients, with an encouraging sensitivity of 91.2% (83.4%-96.1%), whereas microscopy showed 50.6% (39.9%-61.2%) sensitivity. Among all suspected PKDL cases, 42 cases were positive in both microscopy and qPCR, whereas 41 cases were detected as positive through qPCR only. Conclusions: This study provides evidence that real-time PCR is a promising tool for diagnosis of PKDL in endemic regions. In addition to diagnosis, the quantitative ability of this method could be further exploited for after-treatment prognosis and cure assessment of PKDL cases.
- Subject
- diagnosis; microscopy; post-kala-azar dermal leishmaniasis (PKDL); real-time PCR
- Identifier
- http://hdl.handle.net/1959.13/1419919
- Identifier
- uon:37513
- Identifier
- ISSN:2328-8957
- Rights
- © The Author(s) 2018. Published by Oxford University Press on behalf of Infectious Diseases Society of America. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
- Language
- eng
- Full Text
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